Addendum 12/19

Julia: Add addendum and data table onto blog
Kent: Edit SEM photo onto seashore
Christina: Add procedure onto blog

Pollen Data Table

Data Table

Hypothesis

We think the pollen size will grow until it's first bloom(2nd sample), but then decrease once it's fully developed (3rd sample), because it will start to dry out.

Addendum 12/17

Julia: Took pictures and measurements on SEM, added addendum, drew pollen grains on compound microscope.
Kent: Adding pictures to blog, wrote observations of SEM pictures, drew pollen grains using compound microscope.
Christina: added background information to blog, took pictures on SEM, drew pollen grains using compound microscope.

Addendum 12/16

Julia: Add more onto blog (addendum), prepare for SEM, SEM photographer, Data Collector
Kent: Add procedure on blog, prepare for SEM, Recorder, SEM operator
Christina: (Absent) added background info to blog

Background

We watched a video on pollen in vitro involving asian lilies. It showed very rapid pollen tube growth (300 nm/sec) which made us believe that there will be much more distinguished change between our samples. The video shows the pollen grains growing from water absorption in order to make a pollen tube to distribute the sperm down to the ovary. Although we are looking at changes in developed pollen, it's interesting to see the beginning stages of how pollen is originally formed.

the video



This link is a long article on lilies and has lots of details and photographs of all the different parts of lilies, including a little on pollen. this is helpful to our experiment because all of our three plants that we are using are lilies so we need lots of information on lilies.

http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artfeb08/bj-lilies.html



Background information on pollen:
A pollen grain consists of two cells: a generative cell and a tube cell. A hard, resistant wall will form around the two cells in order to complete the pollen grains; the wall is rich in cellulose.  The spore wall of the pollen grains will show the differences in size, shape, and surface markings and can therefore be used to identify the species of a sample.

The image above is an example of how the outer spore wall of pollen grains vary in size, shape, and structure; which is what makes each species distinguishable. 

Source: http://www.vcbio.science.ru.nl/en/virtuallessons/pollendevelopment/

question?

How does pollen morphology change as the plant develops?

Addendum 12/12

Julia Hendrickson: Add requirements on blog (team agreement and addendum), finish google form for station 3
Christina Trang:add requirements on blog (add pictures and procedure) , finish google form for station 3
Kent Ross: add requirements on blog (question, background, and hypothesis), finish google form for station 3

Team agreement

Kent Ross: Created blog, helped take pictures on leica microscope, researched.
Christina Trang: Took pictures of lilies, bought lilies, helped make stub, took pictures on leica microscope, dried the lilies, researched.
Julia Hendrickson: wrote up team agreement, helped make the stub, helped take picture on leica microscope, researched.